Today I basically did the same thing I have been doing the past two weeks. We started another western blott today with our bloodfed dissected mosquitoes and the non-bloodfed mosquitos in order to compare the amount of phosphorylated insulin receptor in the two groups. Today was more exciting because I did a lot of the western blott on my own. I set up and ran the gel and prepared the nitrocellulose paper and most of the western blott sandwich for running. I also took the gel and the paper out, which is one of the hardest parts of the procedure because the gel can stick to the paper and if it dries then you can't read it and it's important not to tear the gel and the paper. I did mess up a little and couldn't get a small piece of the gel off, but luckily it was over a part of the paper that didn't matter too much and it was ok that it didn't come off there. I also did a lot of dissections for the next western blott we will run that I probably will not be able to see.
Over the weekend I got my TB test and background check so that I can shadow in the OR on Friday and I will be shadowing a general surgeon, Dr. Harper. I also worked on my paper a bit over the weekend and Dr. Nuss said that he would read it tomorrow to make sure it's accurate and organized well.
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment