01-14-10: Mosquito dissection day

Today I dissected 144 mosquitos... For our next Western blott we will be testing to see if phosphorolated (active) insulin receptor is present in the Anopholes stephensi mosquito. We will also be testing to see where the receptor is present (the head, thorax and abdomen.) So... today, Dr. Nuss and I collected about 150 mosquitos and anethesized them by putting them on ice. Then we removed the legs and wings and then seperated the head, thorax, and abdomen. I did that basically all day. Then we took what we had collected and grinded it up with a pestel and kept them in a -20 refridgerator. Tomorrow I will be shadowing Dr. Manning.